Demonstration of Virus-like Particles in a Bovine Cell Line

Incorporation of tick-borne encephalitis virus replicons into virus-like particles by a packaging cell line.

RNA replicons derived from flavivirus genomes show considerable potential as gene transfer and immunization vectors. A convenient and efficient encapsidation system is an important prerequisite for the practical application of such vectors. In this work, tick-borne encephalitis (TBE) virus replicons and an appropriate packaging cell line were constructed and characterized. A stable CHO cell lin...

Transient expression of virus-like particles in plants: a promising platform for rapid vaccine production

Transient expression is an efficient and fast system to express recombinant proteins which has been used in different eukaryotic hosts such as mammalian and plant cells. Several applications of this system have so far been used which expression of proteins of interest is one of them. Recently, plants have attracted attention for being used as hosts for the production of recombinant pharmaceutic...

Fine-structural study of R-type virus-like particles in a hamster macrophage cell line

In an attempt to isolate virus particles, a hamster macrophage cell line was examined by electron microscopy. This cell line was established from hamster macrophages infiltrating human lymphoid cells heterotransplanted in Syrian hamsters. The cultured cells appeared to be macrophages, containing round smooth nuclei with clear nucleoli, and numerous cytoplasmic primary lysosomes, polysomes, and ...

Avian lymphoid leukosis: Virus-like particles in kidney Cells

Aids Virus Particles from a Cloned T Cell Line

A unique human retrovirus (RV) 1 has been consistently isolated f rom patients with the acquired immune deficiency syndrome (AIDS). Infection o f human T lymphocytes or T lymphocyte lines with the AIDS RV can result in a variety of outcomes ranging from rapid cell death to the integration of functionally inert proviral DNAs (1-3). A typical AIDS RV infection is characterized by the appearance o...